Regulatory

Part:BBa_K3179002:Design

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-13)
Revision as of 19:24, 19 October 2019 by Bigxushen (Talk | contribs) (→‎References)

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miR 885-5p-target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part, four repeating sequences complementary to miRNA885-5p on an mRNA, is a 4X miRNA target designed to target miRNA885-5p. When miRNA885-5p reaches a certain concentration in the cell, it would combine with miR885-5p-target and suppress the translation of mRNA. According to our design, miR885-5p-target is designed to control the translation of mRNA with response parts. We integrate miR885-5p-target with 2kt-E1A and miR663b-target so that E1A cannot be translated with miRNA885-5p existing in the cell.

Source

We get this sequence from NCBI, and the sequence was synthesized from a biological company.

References

1 Bartel D P. MicroRNAs: target recognition and regulatory functions[J]. cell, 2009, 136(2): 215-233.