Coding

Part:BBa_K2997003

Designed by: Ting-Han Sung   Group: iGEM19_NCKU_Tainan   (2019-10-12)
Revision as of 19:12, 19 October 2019 by Yeohcs88 (Talk | contribs)


Sigma 54-dependent transcriptional regulator (pchR)

The pchR gene can be translated into activator proteins that can bind with the inducer (p-Cresol) and leads to the conformation of the protein. In other words, the activator protein can bind with p-Cresol to form a protein-p-Cresol complex that will then bind onto the p-Cresol sensing region located just downstream of the pchR gene. The binding of the protein - p-Cresol complex initiates the expression of the downstream gene, in our case GFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1738
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal AgeI site found at 179
    Illegal AgeI site found at 1079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1105


[edit]
Categories
Parameters
None