DNA

Part:BBa_K2960000

Designed by: Michelle Croen   Group: iGEM19_Cornell   (2019-10-16)
Revision as of 18:57, 19 October 2019 by Aemetzloff (Talk | contribs)


ssDNA aptamer which binds to microcystin-LR <img class="standard-page-content-image" style = "height: 400px; width: 700px" src="T--Cornell--Translocase.png"> This aptamer was used to create a rapid colorimetric microcystin-detection sensor. Gold nanoparticles (AuNPs) were adsorbed onto the aptamer surface. Normally, in the presence of NaCl, AuNPs will aggregate, shifting the solution color from red to blue. [Show picture] Adsorption onto the aptamer prevents this from happening. However, in the presence of microcystin, the aptamer dissociates from the AuNPs. Thus, they will aggregate when induced by salt, resulting in a shift in the absorption spectra of the solution. We found there to be a log-linear decrease in the absorption at 320 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None