Translational_Unit

Part:BBa_K3165058

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-19)
Revision as of 23:04, 20 October 2019 by Souvadra (Talk | contribs) (→‎Usage and Biology)

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RBS + PcyA

Phycocyanobilin ferredoxin oxidoreductase (PcyA) with a ribosome binding site (RBS).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Biology

The phycocyanobilin ferredoxin oxidoreductase coding sequence (encoding the enzyme for the reduction of biliverdin IX alpha into phycocyanobilins) is coupled with an RBS (BBa_B0034), resulting in the formation of a translational unit. The RBS (with a Shine Dalgarno like sequence) helps in the binding of the ribosome initiating the process of translation.

Usage

This part is used to translate the PcyA gene construct into the functional enzyme under a suitable promoter. In association with the translational unit coding for ho1 (BBa_K3165030), this sequence can act as a genetic circuit for the biosynthesis of phycocyanobilins (PCBs).


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Categories
Parameters
None