Regulatory

Part:BBa_K3171171:Design

Designed by: Yashika Venkatesh   Group: iGEM19_Groningen   (2019-10-16)
Revision as of 18:53, 19 October 2019 by Yashikavenkatesh (Talk | contribs)


Vibrio natriegens native P1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 305
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7
    Illegal SpeI site found at 306
    Illegal PstI site found at 320
    Illegal NotI site found at 13
    Illegal NotI site found at 313
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 7
    Illegal suffix found in sequence at 306
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 7
    Illegal XbaI site found at 22
    Illegal SpeI site found at 306
    Illegal PstI site found at 320
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The strength of the P1 promoter was measured in the P1-mCherry construct, which was designed by 3A assembly method. A synthetic promoter library was constructed by enhancing the function and inducibility. The screening was done based on fluorescence intensity measured. The best variation of the promoter was determined based on fold change in the fluorescence intensity and low basal level.



Source

Source

References