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Part:BBa_J70026:Design

Designed by: Tom Knight   Group: Knight Lab   (2008-10-20)
Revision as of 16:59, 6 May 2009 by Tk (Talk | contribs) (→‎References)

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cat cassette (chloramphenicol resistance)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation in sequence at base 99, ignored. GATC site eliminated at the end by truncation.


GTTTCTTCGAATTCGCGGCCGCTTCTAGAGaatagttacccttattatcaag,J70026-F

GTTTCTTCCTGCAGCGGCCGCTACTAGTAtctggagctgtaatataaaaacc,J70026-R

Source

Genbank V01277, by way of pKV104 from Duncan Krause. Bases 978-2006; missing T in the Krause sequence at base 99 of this sequence.

References

Hahn TW, Mothershed EA, Waldo RH 3rd, Krause DC. Construction and analysis of a modified Tn4001 conferring chloramphenicol resistance in Mycoplasma pneumoniae. Plasmid. 1999 Mar;41(2):120-4. PMID: 10087215

Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol. 1982 May;150(2):815-25. PMID: 6950931