Reporter

Part:BBa_K2943901

Designed by: Dror Hadas   Group: iGEM19_TAU_Israel   (2019-10-14)
Revision as of 22:24, 21 October 2019 by DHadas (Talk | contribs)

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Improved mRFP

This sequence is part of a composite part (BBa_K2943902) that aims to improve an existing composite part (BBa_K608014). The sequence is based on the monomer RFP (mRFP, BBa_E1010) with several mutations inside to optimize expression in our composite part. Mutations were placed in order to optimize the mRNA folding energy (calculated by VienneRNA) to gain more efficient translation initiation and therefore higher expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None