RNA

Part:BBa_K3081107

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 15:02, 19 October 2019 by Lelezhang (Talk | contribs)


a 20bp sequence targeting the M (bottom strand) DnaA box on E.coli oriC

The 20 bp sgRNA targets to the M+(top strand) DnaA box on E.coli genome replication initiation region, OriC. In natural situations, M+ is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to M+ box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None