Part:BBa_K2922029

Lysis protein for colicin-N coding region

Summary

Abbreviated as Nkil, lysis protein for Colicin-N is a 5.6 kDa protein, which is encoded by gene cnl. The lysis protein is required for both Colicin-E1 release and partial cell lysis[1]. (Fig.1)

Fig.1 Nkil helps Colicin-N release and makes partial cell lysis.

Identification

When we were building this circuit, we did the nucleic acid gel electrophoresis experiment to verify. After the circuit is built, we sent the plasmid to sequence, and got the correct sequencing. After new molecular cloning experiments, we did Enzyme-Cut identification to certify the plasmid was correct. We used the XbaI and PstI to cut the plasmid, then we got the target separate fragment-159bp. (Fig.2)

Fig.2 The result of this plasmid cut with enzyme XbaI and PstI.

Reference

[1] A. P. Pugsley, The immunity and lysis genes of ColN plasmid pCHAP4. Molecular & General Genetics Mgg 211, 335-341 (1988).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 118
  
• 1000
  COMPATIBLE WITH RFC[1000]