Coding

Part:BBa_K2983061

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-12)
Revision as of 18:00, 21 October 2019 by TomZaplana (Talk | contribs)


FadX of Punica granatum

This part is an Yarrowia lipolytica codon optimized version of the bifunctional fatty acid conjugase / desaturase (FadX) from Punica granatum (Uniprot Q84UB8).

Usage and Biology

The bifunctional fatty acid conjugase / desaturase FadX (EC: 1.14.19.16) catalyses the conversion of linoleic acid, C18:2 (9Z,12Z) into punicic acid, C18:3 (9Z, 11E, 13Z) (Figure 1).

Figure 1. Conversion of linoleic acid to punicic acid (both incorporated into phosphatidylcholine) by EC: 1.14.19.16.

The Punica granatum (pomegranate) FadX (Pg-FadX) [1] is a 45.828 kDa protein of 395 amino acids. It's activity leads to the accumulation of important quantities of punicic acid in the pomegranate seeds.


References

[1] Hornung E, Pernstich C, Feussner I. Formation of conjugated Delta11Delta13-double bonds by Delta12-linoleic acid (1,4)-acyl-lipid-desaturase in pomegranate seeds. Eur J Biochem (2002) 269, 4852-4859.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None