Coding

Part:BBa_K2983061

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-12)
Revision as of 12:09, 19 October 2019 by Anthor91 (Talk | contribs)


FadX of Punica granatum

PgFadX

Conjugated linolenic acids (CLnAs) are synthetized by bifunctional fatty acid conjugase / desaturase (FadX) enzymes from linoleic acid, C18:2 (9Z,12Z).Thus, to convert it into punicic acid, only the presence of a EC: 1.14.19.16 enzyme is necessary (Figure 1).

Two EC: 1.14.19.16 enzymes were described in the literature: one from pomegranate / Punica granatum (Pg-FadX) and another one from the chinese cucumber / chinese snake gourd / Trichosanthes kirilowii (Tk-FadX, BBa_K2983062).

Figure 1:Conversion of linoleic acid to punicic acid (both incorporated into phosphatidylcholine)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None