Composite

Part:BBa_K3081011

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)
Revision as of 18:25, 21 October 2019 by Smileee (Talk | contribs)


pBAD-dCas9-J23119-R1-

This composite part is the principal design of the inducible CRISPR-based DNA replication interference system, with the 20 bp sgRNA targeting to the R1- DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R1 is a high affinity box for DnaA binding. By blocking the binding of DnaA protein to R1 box, severe arrest and inhibition of genome replication initiation is achieved.

Experiment

For more detailed information, see BBa_K3081058

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 5459
    Illegal NheI site found at 5482
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1470
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 5490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None