Composite

Part:BBa_K3317031

Designed by: erick hernandez   Group: iGEM19_UANL   (2019-10-18)
Revision as of 23:22, 18 October 2019 by Erick419 (Talk | contribs)


BMC shell proteins ABJKN from S. enterica LT2

Propanediol utilization Shell corresponding to the bacterial microcompartment (BMCs) S. enterica LT2 (NC_003197.1) ribosome re-initiation site 1 and ribosome re-initiation site 2 was placed between pduJK and pduN was placed between pduAB and pduJK,this sequence is contained in plasmid pMCY30 generously provided by <a href=https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-017-0685-x#Sec23">Dr. Mimi C. Yung</a> The plasmid contains the proteins pduA (BBa_K3317008) pduB(BBa_K3317009) pduJ (BBa_K3317010) pduK (BBa_K3317011) and pduN (BBa_K3317012)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 41
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1761
    Illegal BamHI site found at 1900
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 41
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 41
    Illegal NgoMIV site found at 1388
    Illegal AgeI site found at 1655
    Illegal AgeI site found at 1691
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None