Composite

Part:BBa_K137076

Designed by: Doug Tischer   Group: iGEM08_Caltech   (2008-08-09)
Revision as of 17:24, 17 October 2008 by Jkm (Talk | contribs)

AHL inducible production of hydrogen peroxide

This part produces pyruvate oxidase (K137069) in response to detecting acylhomoserine lactones.


Usage and Biology

Using the Caltech iGEM [http://2008.igem.org/Team:Caltech/Protocols/H2O2_Production_Assay H2O2 production assay], K137076 in pSB1A2 in JI377 produces 800uM of H2O2 at 1 hour after induction (vs. negligible production for JI377 with an empty plasmid).

K137076.gif


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2741
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


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Categories
Parameters
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