Tag
6xHisB5-B5

Part:BBa_K2984014

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-14)
Revision as of 21:08, 19 October 2019 by SandraSy (Talk | contribs)

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6x His-Tag for protein detection, isolation, purification B5-B5

This part is a part of the Chlamy-HUB-Collection. The His-Tag is an amino acid motif at the C- or N-terminus of proteins. It is one of the most commonly used purification tags with a minimum of six or more consecutive histidine residues. They are able to form complexes with metal ions like Ni2+ or Co2+ making it possible to purify the proteins attached to the his-tag when those transition metal ions are connected on beads. The amino acid sequence is HHHHHH.

This part was designed to be used with the MoClo standard and has B5-B5 overhangs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

Plate_L0-6xHis-Tag_B5-B5_E.coli
Fig.1 - Successful transformation of L0-6xHis-Tag_B5-B5 into E. coli.


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Parameters
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