Regulatory
AR A1-A3

Part:BBa_K2984009

Designed by: Sophia Schiebler   Group: iGEM19_Humboldt_Berlin   (2019-10-13)
Revision as of 18:18, 18 October 2019 by Hermano (Talk | contribs)


AR Promoter A1-A3

This part is a part of the Chlamy-HUB-Collection. Another strong constitutive promoter is the AR promoter that is composed of HSP70A and RBCS2 building a tandem promoter. Therefore the HSP70A (A) promoter is fused upstream of another promoter resulting in a stronger transgene expression (Schroda et al. 2000). The combination of the HSP70A (A) promoter and the RBCS2 (R) promoter building PAR has the positive effect that transcriptional gene silencing (TGS) is strongly reduced making this promoter popular for transgene expression (Schroda et al. 2002). This part was designed for C. reinhardtii


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

The AR Promotor was used for two different paromomycin constructs. The first carries the registry part BBa_K2703008 which we wanted to compare to our improved paromomycin construct which also feautres this promoter. The improvement was done as part of the 2019 gold criteria.

Fig.1 - TAP-agar plates containing paromomycin. The C.Reinhardtii colonies growing on the plate were tranformed with our improved paromomycin construct which is flanked by this AR promoter and the Rbcs2 terminator



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