Composite

Part:BBa_K3040006

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)
Revision as of 13:43, 18 October 2019 by Liya (Talk | contribs)

Description

It is pointed out by the previous iGEM team, UPF CRG Barcelona_2018 iGEM, that the native promoter pFadBA has great leakage and is a weak promoter that isn’t ideal enough for our system. Therefore, to enhance the sensitivity and reduce the leakage of the promoter pFadBA for making it more suitable for our system, we modified the native promoter pFadBA with a mutant in its consensus sequence of -10 and -35 region. According to the previous research [1], there is improvement in expression for pFadBA with consensus sequence.

Furthermore, the improved in expression portion enhance greatly if there’s a point of mutant in consensus sequence. Therefore, we designed our promoter pFadBA with a mutant in its consensus sequence of -10 and -35 region, to promote its strength.

Reference

JENSEN, Peter Ruhdal; HAMMER, Karin. The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters. Appl. Environ. Microbiol., 1998, 64.1: 82-87.

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