Part:BBa_K3160021
MCherry-miR-21-17 sensor
The expression of miR-21 and miR-17 were found to be significantly associated with all tumor stage of gastric cancer. We designed miR-21-17 sensor contain complementary binding sites to miR-21 and miR-17 which inhibit miR-21 and miR-17 expression. The sequences of miR-21-17 sensor were synthesized by GenScript (Shanghai, China). We inserted miR-21-17 sensor into the 3’UTR of mcherry, which generated mcherry -miR-21-17 sensor.
Usage and Biology
The mcherry-miR-21-17 sensor was controlled by EF-1α promoter (EF-1 alpha -mcherry-miR-21-17). The plasmids of EF-1 alpha -mcherry-miR-21-17 were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We measuresd the fluorescence of mcherry in different cells transfected with EF-1 alpha -mcherry-miR-21-17 for 24 h by plate reader (SpectraMax i3), which analyses the potential value of EF-1 alpha -mcherry-miR-21-17 to measure the expression of miR-21 and miR-17.
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