Part:BBa_K3204002
Receptor binding protein of paenibacillus phage HB10c2
This is the putative Receptor binding protein of the paenibacillus phage HB10c2 with the DNA sequence codon-optimized for the expression in E. coli. It binds an unknown receptor on Paenibacillus larvae, the pathogen causing American foulbrood. The binding is very specific to Paenibacillus larvae.
The protein has been identified in silico as the most promising. Modeling of several genes in the cluster of structural proteins of HB10c2 with the webtool Phyre2 has led us to propose the gene order
(...) - tail length tape measure protein (14) - dit (15) - tal (16) - RBP (17) - (unknown proteins) - lysin (21) - (...)
This order resembles well known clusters of baseplate coding regions for other Siphoviridae phages.
The binding has not been proven with sufficiently reliable experimental data. The experiment suggest, that the binding could have worked, but the data is not resilient enough to claim it.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 649
- 1000COMPATIBLE WITH RFC[1000]
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