Coding
Part:BBa_K3224003
Designed by: VIGNESH KUMAR Group: iGEM19_REC-CHENNAI (2019-10-09)
BP100-(KH)₉
Cell-Penetrating Peptides (CPP) are short peptides that can penetrate the cell membrane and deliver a wide range of cargoes into the cell. This part is the coding sequence of our chimeric CPP formed with BP100 and the polycationic peptide (KH)9. It is proven to deliver proteins and nucleic acids into the plant and prokaryotic cells. The polycationic peptide is an efficient nucleic acid carrier and has the ability to promote buffering effect on the pre-lysosomal vesicle. The cargo being negatively charged interacts with the polycationic end of the peptide through ionic interactions, whereas the BP100 region mediates cellular entry through carpet-mechanism.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |