Coding

Part:BBa_K2984040

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 21:10, 18 October 2019 by Chlamy Dima (Talk | contribs)


Bleomycin resistance, B1-B1

This part is a part of the Chlamy-HUB-Collection. Chlamydomonas optimized gene encoding a resitance against the antibiotic bleomycin. The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every two bleomycin molecules, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:2 to the antibiotic (Hayes et al., 1990). By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme (Lumbreras et al. 1998).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 632
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Lumbreras, V., Stevens, D. R., & Purton, S. (1998). Efficient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron. The Plant Journal, 14(4), 441-447.
  2. Hayes, J. D., & Wolf, C. R. (1990). Molecular mechanisms of drug resistance. Biochemical Journal, 272(2), 281.
[edit]
Categories
Parameters
None