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Part:BBa_K2924034

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-16)
Revision as of 13:54, 17 October 2019 by AndreasN (Talk | contribs)


Pcpc560 + α-s1-casein + T1/T7 Terminator

Strong constitutive cyanobacterial promoter Pcpc560 expressing a-s1-casein with the T1/T7 double terminator.

The promoter was cloned into the pSHDY plasmid. The pSHDY plasmid is an RSF1010-based, low-copy self-replicating vector derived from pVZ321 and has a broad host range, which can ensure the conjugation from E. coli to cyanobacteria and other microorganisms1. After testing the strength of the promoter with a fluorescent reporter mVenus, it was used to express a protein from cow’s milk, a-s1-casein in Synechocystis sp. PCC 6803. ===Usage and Biology=== Sequence and Features BBa_K2924034 SequenceAndFeatures

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