Composite

Part:BBa_K2950000

Designed by: Wanji Li   Group: iGEM19_BNDS_China   (2019-10-17)
Revision as of 07:43, 20 October 2019 by CJHH (Talk | contribs)


pYES-GAL1 promoter-OKS-TDH1 terminator-TEF1 promoter-ZhuI-CYC1 terminator

In our study, we aim to achieve carminic acid yield by S. cerevisiae, so we should firstly realize the production of the flavokermesic acid which is an intermediate between acetyl-CoA or malonyl-CoA and carminic acid in the biosynthesis pathway.

At the beginning, we transformed a plasmid with pYES as the copy vector to keep high copy number, the Aloe arborescens octaketide synthase (OKS) to synthesize non-reduced linear octaketide, and the ZhuI cyclase to convert non-reduced linear octaketide to flavokermesic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None