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Part:BBa_K3071013
PspA promoter
Description
This is an regulatory element that originated from Escherichia coli K12 strain.
Biology
![T--Hong Kong-CUHK--pspA promotor structure.png](/wiki/images/4/48/T--Hong_Kong-CUHK--pspA_promotor_structure.png)
pspA promotor is a sigma-54 (σ-54) regulated activator dependent promotor. In the pspA promoter upstream region, it contains σ-54 consensus sequence 5' of the start contains a GG doublet as -24 and a consensus GG doublet at -12, the high-affinity IHF site (-25 to -60), as well as the UAS sites (UAS I: -89 to -107; UAS II:-111 to -129).
Usage
![T--Hong Kong-CUHK--sigma 54 activation.png](/wiki/images/5/5c/T--Hong_Kong-CUHK--sigma_54_activation.png)
sigm54-RNA holoenzyme (σ-54 RNAP) forms an inactive transcriptional initiation complex on this promotor, which can be activated in E. coli by the bacterial enhancer-binding protein PspF (BBa_K3071006). PspF functions by binding to the upstream activation sequences (UAS) near the promoter and contacting the promotor-bound σ-54 RNAP via DNA looping stabilized by the binding of integration host factor (IHF). Previous research has demonstrated the property of pspF-dependent and enhancer-specific transcription activation of pspA promotor.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 12
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |