Coding

Part:BBa_K2984029

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 09:14, 17 October 2019 by Chlamy Dima (Talk | contribs)


L1c-PsaD-GLE-PETase-YFP-SP20-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the GLE secretion signal, the PETase enzyme, a YFP mVenus marker, the SP20 glycomodule for enhanced secretion and the Rbcs2 terminator. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1314
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal XhoI site found at 1038
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1775
    Illegal PstI site found at 1989
    Illegal NgoMIV site found at 2466
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii


[edit]
Categories
Parameters
chassisC. reinhardtii
emission528 nm
excitation515 nm
proteinPETase-YFP