Coding

Part:BBa_K2984027

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 08:43, 17 October 2019 by Chlamy Dima (Talk | contribs)


L1c-Psad-ARS-YFP-Rbcs2

L1c plasmid containg arylsulfatase secretion signal ARS and mVenus fluorescent marker which are flanked by PsaD promoter and Rbcs2 Terminator. The construct is designed to test for the efficency of the secretion signal and the locus dependent secretion via fluorescence intensity measurements.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1110
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1110
    Illegal NgoMIV site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct could then be isolated from E. coli to be transformed in C. reinhardtii



[edit]
Categories
Parameters
chassisC.reinhardtii
emission528 nm
excitation515 nm