Composite

Part:BBa_K3320006

Designed by: Isaac de Paula Guerreiro, Giovanna Maklouf   Group: iGEM19_Amazonas-Brazil   (2019-10-16)
Revision as of 01:28, 21 October 2019 by Jocomodaro (Talk | contribs)


Multi-input AND-gate

This is a multi-input AND-gate for biosensor tumor microenvironment.

Usage and Biology

Our AND Gate has repressor-promoter relation, basically using two promoters to control the signal (repressor) production that represses our protein of interest.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
    Illegal NheI site found at 808
    Illegal NheI site found at 831
    Illegal NheI site found at 4308
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1415
    Illegal AgeI site found at 4057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3284


[edit]
Categories
Parameters
None