Coding
Part:BBa_K2960011
Designed by: Ann Metzloff Group: iGEM19_Cornell (2019-10-16)
Revision as of 00:53, 21 October 2019 by Aemetzloff (Talk | contribs)
The mlrF enzyme
A cassette of enzymes endogenous to Sphingopyxis sp. has been shown to sequentially break down microcystin-LR (the most common and toxic microcystin that results from harmful algal blooms). The enzymes have been named mlrA, mlrB, mlrC, mlrD, mlrE, and mlrF.
MlrF has recently been identified in the genome of Sphingopyxis sp. strain C-1 and may be involved in microcystin degradation; is the expected sixth enzyme in the microcystin-degradation pathway. Its exact function is still unknown but it likely is involved with catalyzing reactions downstream to those of mlrA through C.
Figure 1. Schematic of the sequential degradation of MC-LR by the mlr enzyme cluster. MlrF is circled in red.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
| None |