Coding

Part:BBa_K3059624

Designed by: Adam Oliver   Group: iGEM19_William_and_Mary   (2019-10-16)
Revision as of 00:59, 17 October 2019 by Ajoliver (Talk | contribs)

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EL222

Protein with a blue light reactive LOV domain that exposes a DNA binding domain when activated. Allows for repression or activation of genes depending on the used promoter sequence.

Sequence was codon optimized using Twist's codon optimization tool.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 502
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None