Coding

Part:BBa_K3183202:Design

Designed by: Natasha Cooke   Group: iGEM19_Oxford   (2019-10-16)
Revision as of 03:16, 22 October 2019 by Mrfelf (Talk | contribs) (→‎References)

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pET28A-AgrBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is optimised for E. coli and contains a His tag after each protein to allow individual purification.


Source

C. difficile genomic data.

References

Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.