Coding

Part:BBa_K2558003:Experience

Designed by: Tianze Huang   Group: iGEM18_Tsinghua   (2018-10-02)
Revision as of 22:08, 16 October 2019 by AlexNiederau (Talk | contribs) (→‎dCas9 Fluorescence Loss Assays)


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dCas9 Fluorescence Loss Assays

Figure1: Fluorescence Loss Assay. Amount of GFP fluorescence under the induction of dCas9 targeting GFP.

The expression of dCas9 is regulated by an IPTG inducible expression system. Sp1 - 3 represent three different spacers targeting GFP or the associated promoter. SpNT represents a non-targeting spacer acting as a control.

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