Part:BBa_K3016200:Design
Vibrio natriegens' TorA signal peptide containing YGFP gene
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 781
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
-
Source
TorA signal peptide sequence from Vibrio natriegens ATCC 14048 / DSM 759 genomic sequence, NCBI ID NZ_CP016345.1 & NZ_CP016346.1. YGFP gene from Weinstock et al., 2016, supplementary data (see references).
References
Alanen, H. I., Walker, K. L., Suberbie, M. L. V., Matos, C. F., Bönisch, S., Freedman, R. B., ... & Robinson, C. (2015). Efficient export of human growth hormone, interferon α2b and antibody fragments to the periplasm by the Escherichia coli Tat pathway in the absence of prior disulfide bond formation. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1853(3), 756-763.
Jong, W. S., Vikström, D., Houben, D., de Gier, J. W., & Luirink, J. (2017). Application of an E. coli signal sequence as a versatile inclusion body tag. Microbial cell factories, 16(1), 50.
Sochacki, K. A., Shkel, I. A., Record, M. T., & Weisshaar, J. C. (2011). Protein diffusion in the periplasm of E. coli under osmotic stress. Biophysical journal, 100(1), 22-31.
Weinstock, M. T., Hesek, E. D., Wilson, C. M., & Gibson, D. G. (2016). Vibrio natriegens as a fast-growing host for molecular biology. Nat Methods, 13(10), 849-851. doi:10.1038/nmeth.3970