Composite

Part:BBa_K3016200:Design

Designed by: Antti Pennanen, Antti Toivanen   Group: iGEM19_Aalto-Helsinki   (2019-10-16)
Revision as of 21:21, 16 October 2019 by Anttipen (Talk | contribs) (→‎Source)


Vibrio natriegens' TorA signal peptide containing YGFP gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 781
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

TorA signal peptide sequence from Vibrio natriegens ATCC 14048 / DSM 759 genomic sequence, NCBI ID NZ_CP016345.1 & NZ_CP016346.1. YGFP gene from Weinstock et al., 2016, supplementary data (see references).

References

Weinstock, M. T., Hesek, E. D., Wilson, C. M., & Gibson, D. G. (2016). Vibrio natriegens as a fast-growing host for molecular biology. Nat Methods, 13(10), 849-851. doi:10.1038/nmeth.3970