Coding

Part:BBa_K2984052

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 12:46, 17 October 2019 by Chlamy Dima (Talk | contribs)


L1c-PsadIntron-bleRscp-ARS-YFP-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. Level 1 construct containing secretion signal of arylsulfatase ARS fused to the flourescent protein mVenus. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the PsaDintron. The construct ends with the Rbcs2 terminator. Sequence and Features BBa_K2984052 SequenceAndFeatures ==Characterization==

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii


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