Coding
BSAL

Part:BBa_K3051005

Designed by: Megha Bawa   Group: iGEM19_Warwick   (2019-10-13)
Revision as of 19:58, 16 October 2019 by AleColosio (Talk | contribs)


Bacillus Subtilis Lipase

This is a lipase from Bacillus Subtilis XF-1 it was found in during a metagenomic analysis of restaurant wastewater containing high levels of FOG (fat oil and grease). It has a kDa of 24.1.

It was tested and characterized using a p nitrophenol octanoate assay. Details of this assay are available on the iGem 2019 wiki page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The part contains a EstA triacylglycerol lipase conserved region, which makes up a large proportion of its total length.


[edit]
Categories
Parameters
None