Regulatory

Part:BBa_K3002027

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-16)
Revision as of 18:52, 16 October 2019 by MSchlosser (Talk | contribs)

pAR promoter for Chlamydomonas reinhardtii (Phytobrick) >html> This basic part contains the PAR promoter and a 5'UTR (A1-B2) for Chlamydomonas reinhardtii and was built as a part of the Kaiser Collection. Combined with a CDS, and a terminator, this level 0 construct leads to a high expression of a target protein. Especially in combination with the part <a href="https://parts.igem.org/Part:BBa_K3002000">BBa_K3002000</a>(Spectinomycin resistance gene aadA) a high expression can be observed. As this part is a combination of the promoters HSP70A (upstream) and RBCS2, no heat shock is needed as a transcription activator (<a href="https://doi.org/10.1046/j.1365-313x.2000.00652.x"> Schroda, M.,2000</a>). </html>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None