Composite
Part:BBa_K2948005
Designed by: Zhang Xinyu Group: iGEM19_GZHS-United (2019-10-16)
Heme Oxygenase with GroE & RBS (E. coli Codon Optimised)
Hemeoxygenase with RBS (E. coli Codon Optimised), sequences obtained from BBa_K953000, and we turned the T7 promoter(Part BBa_I712074) into GroE promoter (BBa_K763001) This complex can then absorb red light (620-750 nm) to excite the bacteriophytochrome and result in a phenotypic change from blue to green. This can be reversed by far-red light (700-800 nm) or will revert from green to blue over time. As E. coli does not produce biliverdin, heme oxygenase must be coupled with a bacteriophytochrome to activate the oxidation of heme to produce biliverdin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 312
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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