DNA

Part:BBa_K3053001

Designed by: Rajeeva Lokshanan   Group: iGEM19_VIT_Vellore   (2019-10-16)
Revision as of 16:02, 21 October 2019 by LokshananR (Talk | contribs)

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RBS + UTR of beta lactamase

This part consists of the RBS with the UTR that targets the beta lactamase mRNA and represses its expression by forming a double stranded mRNA.


Usage and Biology

The antisense region is flanked by an RBS sequence on the 5' end and a complementary RBS sequence on the 3' end. The RBS forms a duplex by binding to its complementary RBS sequence in the absence of antibiotic resistance gene & thus, inactivates the expression of the Cro gene. When the antibiotic resistant gene is present, the RBS binds to the complement RBS in the mRNA of the resistant bacterium & enables the expression of the Cro gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None