Device
Part:BBa_K2940014:Design
Designed by: Jakub Chromy Group: iGEM19_Edinburgh_OG (2019-10-16)
XylR coding device-sfGFP_optimism coding device with Pu
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2446
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2101
Illegal XhoI site found at 3018 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
In an attempt to improve the sensitivity of the biosensor, the codon-optimized superfolder GFP was used as the reporter.
Source
The part is identical to BBa_K2023015 part developed by Ionis Paris iGEM team 2016, except for the originally used wild-type GFP (BBa_E0040) reporter gene being replaced by codon-optimized superfolder GFP (BBa_K2541400).