Device
Part:BBa_K2940014
Designed by: Jakub Chromy Group: iGEM19_Edinburgh_OG (2019-10-16)
Revision as of 21:39, 20 October 2019 by Jakub Chromy (Talk | contribs)
XylR coding device-sfGFP_optimism coding device with Pu
This part encodes the biosensor circuit comprising the toluene-specific transcription factor XylR and the sfGFP reporter gene.
The part was meant to serve as the starting biosensor circuit platform for the subsequent directed evolution of XylR in order to develop a biosensor for the detection of aromatic amines.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2446
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2101
Illegal XhoI site found at 3018 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 329
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/gfp
//cds/transcriptionalregulator/activator
//function/reporter/fluorescence
//cds/transcriptionalregulator/activator
//function/reporter/fluorescence
chassis | Escherichia coli TOP10 |
device_type | Biosensor |
emission | 509 nm |
excitation | 490 nm |
proteins | XylR BBa_K2023005, sfGFP BBa_K2541400 |