DNA
Part:BBa_K2940007
Designed by: Ping CHANG Group: iGEM19_Edinburgh_OG (2019-10-16)
Secreted dye-degrading peroxidase(DyP)
This part is designed as the control group to check whether the original DyP can be expressed and degrade dyes. So the coding sequence was refrenenced from NCBI () without any modification. The strong constitutive promoter and RBS combination were introduced to make the DNA construct. It was cloned into pSB1C3 using classcial cloning and was verified using ABTS assay.
Dye-degrading peroxidase is from Bacillus subtilis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 10
Illegal NheI site found at 33 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 155
Illegal BsaI site found at 164
Illegal BsaI site found at 1130
Illegal SapI site found at 560
Illegal SapI site found at 918
Illegal SapI.rc site found at 1074
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Categories
Parameters
None |