Plasmid
Part:BBa_K3179105:Design
Designed by: Jiaxian Xu Group: iGEM19_SYSU-CHINA (2019-10-16)
pT2mKE
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 999
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 203
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 999
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 999
Illegal NgoMIV site found at 473
Illegal AgeI site found at 209 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
pTRE-mi663b-2Kt-E1-miR885-5p
Source
pTRE-mi663b-2Kt-E1-miR885-5p
References
1 Wroblewska L, Kitada T, Endo K, et al. Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery[J]. Nature biotechnology, 2015, 33(8): 839. 2 Berk A J. Functions of adenovirus E1A[J]. Cancer surveys, 1986, 5(2): 367-387.