Composite

Part:BBa_K118024:Design

Designed by: Andrew Hall   Group: iGEM08_Edinburgh   (2008-10-07)
Revision as of 14:43, 7 October 2008 by Andhi (Talk | contribs) (→‎References)

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dxs+LIMS1+appY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 746
    Illegal SapI.rc site found at 2006


Design Notes

The order of the genes in the operon corresponds to our perceived importance of each gene, bearing in mind that translation is more efficient of the genes early in the mRNA.


Source

dxs and appY are both from E. coli JM109 genomic DNA. lims1 is from Citrus limon genomic DNA.

References

Kang, M. J. et al. (2005) Identification of Genes Affecting Lycopene Accumulation in Escherichia coli Using a Shot-Gun Method. Biotechnology and Bioengineering 91(5): 636-642.