Composite

Part:BBa_K3196031

Designed by: Hao Qiu   Group: iGEM19_HUST-China   (2019-10-16)
Revision as of 12:47, 20 October 2019 by Orange-Huo (Talk | contribs)


pGAP-Kozak-FLO10 pro-pelA-His tag-TT-Pgpda-Kozak-FLO10-SLAC-His tag-TT-Pgas-Alkali-TT


We combine pelA, SLAC genes, which each adds His tag behind together.Through the previous experiment, we find the signal peptide which is most suitable for the target genes, and form the final pathway.Besides we add an alkali peptide to maintain pH.

Characterization

This is a three section for degrade banana fiber part.

Usage and Biology

We put pectinase pelA at the first place because the banana fiber is covered by pectin. After dealing with the surface of the fiber, SLAC is as follow to degrade lignin inside the banana further, aiming to gain higher quality of the fiber. Since all these three enzymes’ optimum pH is near 5, we designed a pH system. We use Pgas, a promoter activate at pH equals 5, matching the optimum pH of the enzymes. In case the whole system’s pH decreases too much, we use Pgpda, a promoter activate at pH equals 2, connecting it with an alkali peptide to stabilize pH.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1527
    Illegal BamHI site found at 478
    Illegal BamHI site found at 2346
    Illegal BamHI site found at 2357
    Illegal BamHI site found at 2368
    Illegal BamHI site found at 3720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2807
    Illegal SapI site found at 238


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Parameters
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