Part:BBa_K3253003
Ghchi 6
Aphid is one of the most notorious economic pests in the world. They feed on phloem sap of the host plant and deprive the plant’s nutrients. They have a great impact on the plant’s yield. It is hard to control this kind of pest due to their small size and strong fertility. In addition, aphids control now rely on chemicals like pesticides that may allow the development of resistance. As a result, the aphids are harder to be controlled. Thus, developing a new way to control the aphid and promote yield is a general goal. So we introduce the aphid-resistance gene Ghchi6.
This gene is found in red chicken-feet cotton, and it belongs to Glyco-hydro-19's subfamily. This gene is composed of 975 bases and codes for the synthesis of class I chitinase which is made up of 324 amino acids.Its molecular weight is 34.65 k Da and isoelectric point is 7.81. Through amino acid sequence comparison, cotton gene Ghchi6 and other plant gene are highly conservative, and is evolutionarily close relative to chitin of woody cotton.
When the cotton is physically attacked by aphids, the gene Ghchi6 gets overexpressed that the plant starts to secrete chitinase. The overexpression of Ghchi6 can improve the aphid-resistance in cotton, and we plan to transfer this favored gene to tobacco to improve its aphid-resistance. Through modifications we have transferred the gene to Agrobacterium and then to a common tobacco Nicotiana rustica through infection. Normally the expression of chitinase in plants is very low under common conditions, but in cottons the expression is triggered by external factors like mechanical damage.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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