Translational_Unit
Part:BBa_K118018:Design
Designed by: Andrew Hall Group: iGEM08_Edinburgh (2008-10-06)
rbs+glgC16 (glgC with G336D substitution)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 210
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No special considerations
Source
Escherichia coli JM109 genomic DNA.
References
- Leung, P., Lee, Y. M., Greenberg, E., Esch, K., Boylan, S. and Preiss, J. (1986). Cloning and expression of the Escherichia coli glgC gene from a mutant containing an ADPglucose pyrophosphorylase with altered allosteric properties. J. Bacteriol. 167, 82-88. - [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=212844&blobtype=pdf link]
- Meyer, C. R., Bork, J. A., Nadler, S., Yirsa, J. and Preiss, J. (1998). Site-Directed Mutagenesis of a Regulatory Site of Escherichia coli ADP-Glucose Pyrophosphorylase: The Role of Residue 336 in Allosteric Behavior. Archives of Biochemistry and Biophysics 353, 152-159. - [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-45KKRWW-9P&_user=809099&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000043939&_version=1&_urlVersion=0&_userid=809099&md5=aa8d0cb9ae204115911c188cbdc31b16f link]