Coding
Part:BBa_K118016:Design
Designed by: Andrew Hall Group: iGEM08_Edinburgh (2008-10-06)
glgC16 (glgC with G336D substitution)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 194
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) using the mutagenesis by blunt-ended ligation (MABEL) technique. The genomic DNA sequence also contains to EcoRI sites, both of which have been mutated out using MABEL.
Source
Escherichia coli JM109 genomic DNA