Composite

Part:BBa_K3286044

Designed by: Sebastiaan Kuiper   Group: iGEM19_Wageningen_UR   (2019-10-16)
Revision as of 12:57, 18 October 2019 by SJK (Talk | contribs)


Bacteriophage Lambda PL, PRM and PR promoters, operators and 5' UTR's

The native bacteriophage Lambda promoters (PL, PRM and PR), operator regions (OL1,2,3 and OR1,2,3) and 5' UTR (with RNase III sensitive hairpin-loop and NutL site). The PL and PR promoter can be regulated by the CI and the CRO protein, which is a native lambda transcribed protein.


Ptashne, M. (2004). A genetic switch: phage lambda revisited (3rd ed.). New York: Cold Spring Harbor Laboratory Press.

Wilson, H. R., Kameyama, L., Zhou, J. G., Guarneros, G., & Court, D. L. (1997). Translational repression by a transcriptional elongation factor. Genes and Development, 11(17), 2204–2213. https://doi.org/10.1101/gad.11.17.2204

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None