Regulatory

Part:BBa_K3286043

Designed by: Sebastiaan Kuiper   Group: iGEM19_Wageningen_UR   (2019-10-16)
Revision as of 16:41, 16 October 2019 by SJK (Talk | contribs)


Native bacteriophage Lambda PR and PRM promoter, OR operators and 5' UTR

The native bacteriophage Lambda PR and PRM promoter of bacteriophage Lambda, with the operator regions OR1, OR2 and OR3, and the PR 5'UTR.

The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None