Coding

Part:BBa_K2984037

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-16)
Revision as of 10:11, 17 October 2019 by Chlamy Dima (Talk | contribs)


L1c-Psad-PtxD-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (PtxD) which allows C.reinnhardtii to grow on phosphite. The trancriptional unit starts with the PsaD promoter and Ends with the Rbcs2 terminator. The construct contains a B2-B2 linker which connects the PsaD promoter to PtxD. Sequence and Features BBa_K2984037 SequenceAndFeatures ==Characterization==

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii


[edit]
Categories
Parameters
chassisC.reinhardtii